Research use only — not for human consumption. Not evaluated by the MHRA or FDA.
Peptides
IGF-1 LR3 1mg
1 mg · Lyophilised powder
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| Form | Lyophilised powder |
|---|---|
| Purity specification | ≥ 99% by HPLC (supplier specification) |
| Storage | −20°C lyophilised · 2–8°C once reconstituted |
Research Overview
What IGF-1 LR3 is
IGF-1 LR3 (Long R3 Insulin-like Growth Factor-1) is an 83-residue recombinant analogue of human IGF-1. It differs from the native 70-residue protein in two ways: an arginine replaces glutamate at position 3, and a 13-residue extension is added at the N-terminus. Its molecular weight is approximately 9.1 kDa. These modifications substantially reduce its affinity for IGF-binding proteins, which in native IGF-1 sequester most of the circulating molecule.
Research background
IGF-1 LR3 is a well-established cell-culture reagent. It is widely used as a mitogenic supplement in serum-free and low-serum media, particularly in the culture of CHO and other mammalian cell lines used for recombinant protein production, where its reduced binding-protein interaction provides more predictable activity than native IGF-1. It is also used in signalling studies of the IGF-1 receptor pathway. Megalab supplies it for laboratory use only.
Specification
Each vial contains 1mg of IGF-1 LR3 as a lyophilised powder. Because the compound is a protein rather than a short peptide, the fill weight is lower than other products in the range while still providing ample material for typical cell-culture concentrations. Purity is verified by HPLC at ≥99% on every batch; a Certificate of Analysis is available on request.
Handling and storage
Store unopened vials at −20°C, protected from light. Allow to reach room temperature before opening. Reconstitute with bacteriostatic water or sterile buffer added gently down the vial wall; do not vortex, as proteins of this size can denature under shear. Aliquot the reconstituted solution to avoid repeated freeze–thaw cycles and store at 2–8°C for short-term use, within 30 days.
Supplied for laboratory research use only.
Reconstitution & storage
- Diluent
- Bacteriostatic water, added slowly down the inside wall of the vial; swirl, do not shake.
- Lyophilised
- Store at −20°C, away from light and moisture.
- Reconstituted
- Refrigerate at 2–8°C.
Frequently Asked Questions
What does “LR3” stand for?
Long R3: “Long” refers to the 13-residue N-terminal extension and “R3” to the arginine substitution at position 3. Together they reduce binding to IGF-binding proteins.
Why is the vial only 1mg?
IGF-1 LR3 is a 9.1 kDa protein used at low concentrations in cell culture. One milligram provides sufficient material for a large number of typical culture applications.
Can IGF-1 LR3 be vortexed to dissolve it?
No. Vortexing can denature proteins of this size. Add diluent gently and swirl, or allow the powder to dissolve without agitation.
