Research Guidance · Laboratory Protocol

Reconstituting & Storage

Proper reconstitution and storage is essential to maintaining peptide integrity for research use. Follow these protocols to ensure consistent, reliable results.

How to Reconstitute Your Peptides

01

Allow the vial to reach room temperature

Remove the vial from cold storage and allow it to reach room temperature before opening. This prevents condensation from forming inside the vial, which can compromise the lyophilised powder.

02

Use bacteriostatic water (BW)

Always use bacteriostatic water for reconstitution. It contains 0.9% benzyl alcohol which inhibits bacterial growth, making it suitable for multi-use vials. Avoid distilled or sterile water for long-term storage use.

03

Inject slowly along the inside wall

Draw the required volume of bacteriostatic water into your syringe, then inject slowly along the inside wall of the vial — do not inject directly onto the lyophilised powder as this can damage the peptide structure.

04

Gently swirl — do not shake

Once the water has been added, gently swirl the vial in a slow, circular motion until fully dissolved. Do not shake or vortex — vigorous agitation can damage peptide bonds.

05

Store reconstituted vials in the refrigerator

Once reconstituted, store at 2–8°C. Label with the date of reconstitution and use within 30 days. Never freeze a reconstituted vial.

Temperature & Storage Guidelines

Lyophilised (Freeze-Dried)

-20°C or below

Unopened lyophilised vials should be stored at -20°C or lower in a sealed container away from light and moisture. Shelf life is typically 24+ months when stored correctly.

Reconstituted (In Solution)

2–8°C

Once reconstituted, store in a refrigerator at 2–8°C. Use within 28–30 days. Avoid repeated temperature fluctuations.

Do’s & Don’ts

Do

  • Allow vials to reach room temperature before opening
  • Use bacteriostatic water for reconstitution
  • Inject water slowly along the inside vial wall
  • Swirl gently until fully dissolved
  • Label vials with the reconstitution date
  • Store lyophilised vials at -20°C or below
  • Store reconstituted vials at 2–8°C
  • Work in a clean environment

Don’t

  • Inject water directly onto the lyophilised powder
  • Shake or vortex the vial
  • Freeze reconstituted peptides
  • Use distilled water for long-term storage
  • Expose vials to direct sunlight or heat
  • Repeatedly heat and cool reconstituted vials
  • Use reconstituted peptides after 30 days
  • Open cold vials without allowing them to warm first

Common Reconstitution Volumes

Common bacteriostatic water volumes and resulting concentration per 0.1ml (10 units on an insulin syringe) for reference in controlled in-vitro research settings.

Peptide AmountBW Volume AddedConcentration per 0.1ml (10 IU)
5mg1ml500mcg
5mg2ml250mcg
10mg2ml500mcg
10mg4ml250mcg
50mg5ml1000mcg

Use our Peptides Calculator for precise calculations.

Quick Reference

Lyophilised storage-20°C
Reconstituted storage2–8°C
Use within30 days
Freeze reconstituted?Never
Reconstitution solventBac. Water
Mixing methodSwirl only

Peptides Calculator

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⚠ Research Use Only This guide is for controlled in-vitro research planning only. All Mega Lab Peptides products are for scientific research use only — not for human consumption.

★ IMPORTANT NOTICE

Research Use Only

All products sold by Mega Lab Peptides are strictly for scientific research purposes only. They are not medicines, supplements, or for human consumption. They have not been evaluated or approved by the MHRA or FDA.

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